Genetic Signatures’ FluA assay

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A newly emerging variant of Influenza A (FluA) H3N2, referred to as Subclade K, formerly J.2.4.1, has been increasing in prevalence in the Northern Hemisphere since mid-2025. ^1,²

In-silico alignment confirms that our EasyScreen™ Respiratory Pathogen Detection Kit (RP007) will efficiently detect all currently known sequences of the FluA H3N2 Subclade K variant.

Our unique approach:

Pan-Influenza A targeting of the Matrix Protein gene, avoiding the highly variable Hemagglutinin (H) and Neuraminidase (N) regions.
Powered by 3base^® technology, the assay is more resilient to common mutations, ensuring robust detection of emerging variants. ³

In-silico Assessment of Influenza A Subclade K

A total of 330 Influenza A H3N2 clade 3C.2a1b.2a.2a.3a.1 sequences collected in the United Kingdom between 01 August 2025 and 18 November 2025 were obtained from GISAID ⁴ and aligned with the EasyScreen™ Respiratory Pathogen Detection Kit (RP007) following 3base^® conversion.

317 sequences (96%) showed a perfect (100%) match.
13 sequences (4%) contained a single mutation mismatch in one primer region. The location of these mismatches is not expected to impact the assay’s capability of detecting the Influenza Subclade K variants.

Therefore, 100% of the analysed sequences of the emerging Subclade K variant remain within the expected detection range of Genetic Signatures’ EasyScreen™ Respiratory Pathogen Detection Kit.

EasyScreen™ Respiratory Pathogen Detection Kit (RP007)

The EasyScreen™ Respiratory Pathogen Detection Kit (RP007-HT) is a highly sensitive multiplex real-time PCR assay that detects 14 respiratory pathogens in a single test. The test supports our laboratory partners to deliver rapid, accurate results to support appropriate patient management.

Genetic Signatures also provides an expanded range of respiratory infections, and other leading infectious diseases. Learn more or contact your local representatives by clicking the links below:

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EasyScreen™ Respiratory Pathogen targets
(RP007-HT)

Viruses
Influenza A
Influenza B
Respiratory syncytial virus A/B
Rhinovirus
Human Metapneumovirus
Parainfluenza 1/3
Parainfluenza 2
Enterovirus
Adenovirus

Bacteria
Bordetella pertussis / Bordetella parapertussis
Mycoplasma pneumoniae

The EasyScreen Respiratory Pathogen Detection Kit, comprising a branded box with laboratory tubes in front, each with different lid colours.

We gratefully acknowledge and thank the Authors, Originating Laboratories, and Submitting Laboratories contributing sequence and metadata shared through GISAID, which enabled this analysis ⁴.