EasyScreen™ Workflow

Sample Preparation Workflow:

The EasyScreen™ Sample Processing Kits offer streamlined preparation of nucleic acids directly from the primary specimen.

The kits isolate both DNA and RNA and are compatible with all EasyScreen™ Pathogen Detection Kits.

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Automated NA Extraction Platform Compatibility
Genetic Signatures GS1
bioMérieux NucliSENS easyMAG
Qiagen QIAsymphony, EZ1
Roche MagNA Pure 96
Thermo KingFisher Flex

Once the sample has been prepared using an EasyScreen™ Sample Processing Kit any (or all) of the applicable EasyScreen™ Pathogen Detection Assays can be set up as per the Real-Time PCR workflow below.

For example faecal samples are prepared by dipping a swab into the sample and inoculating the EasyScreen™ Reagent and proceeding as above. The extraction process via  automated nucleic acid extraction yields sufficient eluate to perform all of the bacterial, viral and protozoan PCRs.

Real-Time PCR Workflow:

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Real-Time PCR Platform Compatibility
Bio-rad CFX (96 and 384 well formats)
Roche LC480 (96 and 384 well formats)
Qiagen RotorGene
Applied Biosystems 7900HT / 7500
Applied Biosystems QuantStudio™ 5
Stratagene/Agilent Mx