Enteric Pathogen Screening Kits
Gastrointestinal disease can be caused by a wide range of infectious agents including viral, bacterial and protozoan. Thus, the diagnosis of infectious gastrointestinal disease can be challenging and involve specialists in microbiology, virology, and parasitology.
It has been estimated that there are 16.8 million gastroenteritis cases per year in Australia resulting in 250,000 visits to hospital Emergency Departments, 15,000 hospitalisations and 80 deaths .
In order to simplify the detection of causative agents of infectious gastrointestinal disease we have developed EasyScreen™ real-time PCR screening assays for all major gastroenteritis pathogens. All assays share a universal sample processing method and incorporate our previously described 3base™ technology .
Conventional gastroenteritis diagnosis can in some instances take up to 5 days  to provide a definitive result. To reduce this our assays offer sample to result turnaround time in as little as 3 hours.
Genetic Signatures EasyScreen™ Enteric Pathogen Detection kits offer the following advantages:
- An integrated automatable sample processing method
A complete workflow is offered from clinical specimen to reportable result. In this way, results are also standardised from lab to lab.
- Rapid detection
From specimen to result in 3-5 hours, with minimal hands-on time, compared to days for some traditional methods
- In-built controls
High level of confidence in results as extraction failure and inhibition controls reduce the likelihood of false negative results
- Real-Time PCR format
No post-PCR handling, virtually eliminating potential for contamination
- Thorough coverage of common pathogens
Up to 22 gastroenteritis causing pathogens are simultaneously detected from the one specimen preparation
- Open platform
Compatible with most automated sample preparation systems and real-time PCR instruments commonly found in pathology laboratories
The EasyScreen™ Enteric Pathogen Detection Kits include:
|Product#||EasyScreen™ Kit||Microorganisms Detected|
|EB001 & EB001-HT||Enteric Bacteria Detection||(i) Salmonella spp.
(ii) Campylobacter spp.
(iii) Shigella spp./Enteroinvasive E.coli (EIEC)
(iv) Yersinia enterocolitica.
(v) toxigenic C. difficile and
(vi) Listeria monocytogenes
|EP001 & EP001-HT||Enteric Protozoan Detection||(i) Cryptosporidium spp.,
(ii) Giardia intestinalis,
(iii) Dientamoeba fragilis,
(iv) Entamoeba histolytica and,
(v) Blastocystis hominis
|EV002 & EV002-HT||Enteric Viral Detection||(i) Norovirus GI
(ii) Norovirus GII
(iv) Rotavirus A
(viii) Adenovirus and
(ix) Adenovirus (40 + 41 only)
|CDD001||C. difficile Detection||Toxigenic C. difficile (targets both tcdA and tcdB)|
|CDD002||C. difficile Reflex||Hypervirulent C. difficile incl. ribotype 027 & 078 targeting:
(i) tcdC gene deletion at position 117,
(ii) binary toxin gene (cdtA) and
(iii) gyrA gene mutation (fluoroquinolone resistance)
All kits are sufficient for 100RXNs or 500RXNs for HT (384 well format) kits.
All EasyScreen™ Enteric Pathogen Detection Kits are for use with nucleic acids that have been prepared using EasyScreen™ Sample Processing Kits.
For more information about the EasyScreen™ Workflow and Compatibility with Nucleic Acid Extraction and Real-Time PCR platforms visit our EasyScreen™ Workflow page.
 Source: Australian Government Department of Health and Aging, “The Annual Cost of Foodborne illness in Australia”, March 2006
 Baleriola C, Millar D, Melki J, Coulston N, Altman P, Rismanto N and Rawlinson W. 2008. Comparison of a novel HPV test with the Hybrid Capture II (hcII) and a reference PCR method shows high specificity and positive predictive value for 13 high-risk human papillomavirus infections. J. Clin. Virol. 42:22-6 2008
 Cunningham SA, Sloan LM, Nyre LM, Vetter EA, Mandrekar J and Patel R. 2010. Three-hour molecular detection of Campylobacter, Salmonella, Yersinia and Shigella species in feces with accuracy as high as that of culture. J. Clin. Micro. 48: 2929-33