Sample Preparation Workflow:
The EasyScreen™ Sample Processing Kits offer streamlined preparation of nucleic acids directly from the primary specimen.
The kits isolate both DNA and RNA and are compatible with all EasyScreen™ Pathogen Detection Kits.
|Automated NA Extraction Platform Compatibility|
|Genetic Signatures GS1|
|bioMérieux NucliSENS easyMAG|
|Qiagen QIAsymphony, EZ1|
|Roche MagNA Pure 96|
|Thermo KingFisher Flex|
Once the sample has been prepared using an EasyScreen™ Sample Processing Kit any (or all) of the applicable EasyScreen™ Pathogen Detection assays can be set up as per the Real-Time PCR workflow below.
For example faecal samples are prepared by dipping a swab into the sample and inoculating the EasyScreen™ Reagent and proceeding as above. The extraction process via automated nucleic acid extraction yields sufficient eluate to perform all of the bacterial, viral and protozoan PCRs.
Real-Time PCR Workflow:
|Real-Time PCR Platform Compatibility|
|Bio-rad CFX (96 and 384 well formats)|
|Roche LC480 (96 and 384 well formats)|
|Applied Biosystems 7900HT / 7500|
|Applied Biosystems QuantStudio™ 5 & 7|
More information regarding the specific conditions for each Automated Nucleic Acid Extraction and Real-Time PCR Platform is available on our resources page.