Methylation Publications

Methylation analysis published in the Journal of Translational Psychiatry

Journal of Veterinary and Medical Science

The Plant Cell: American Society of Plant Biologists

Research Article published in Breast Cancer Research & Treatment

Published in Scientific Reports

Frontiers in Microbiology: Virology

Landmark MethylEasy™ Technology application publication

The History & Development of MethylEasy™ Technology

  • Frommer M, Mcdonald C, Millar DS et al (1992).  A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands.  Proc.Nat.Acad.Sci., USA 89: 1827-1831.
  • Strizaker, C, Millar DS, Paul CL, Warnecke PM, Harrison J, Dryja TP, Vincent PC, Frommer M and Clark SJ (1997).  Extensive DNA methylation spanning the RB promoter in retinoblastoma Tumours.  Canc. Res57: 2229-2237.
  • Warnecke PM, Strizaker C, Melki JRMillar DS, Paul CL and Clark SJ (1997).  Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite treated DNA. Nuc. Acids Res25: 4422-4426.
  • Melki, J. R., Warnecke, P., Vincent, P. C., and Clark, S. J. (1998). Increased DNA methyltransferase expression in leukaemia. Leukemia, 12: 311-316.
  • Huschtscha, L. I., Noble, J. R., Neumann, A. A., Moy, E. L., Barry, P., Melki, J. R., Clark, S. J., and Reddel, R. R. (1998). Loss of p16INK4 ex- pression by methylation is associated with lifespan extension of human mammary epithelial cells. Cancer Res, 58: 3508-3512.
  • Millar, D. S., Ow, K., Paul, C. P., Russell P. J., Molloy, P.L., and Clark, S.J. (1999).  Detailed methylation analysis of the Gluthatione S-transferase (GST-P1) gene in prostate cancer.  Oncogene.  18(6): 1313-24.
  • Molloy, P.L., Douglas Millar, Pamela Russell and Susan Clark.  Prostate Cancer DNA Methylation Assay.  Today’s Life Sciences (October 1999).
  • Melki, J. R., Vincent, P. C., and Clark, S. J. (1999). Cancer-specific region of hypermethylation identified within the HIC1 putative tumour suppressor gene in acute myeloid leukaemia. Leukemia, 13: 877- 883.
  • Melki, J. R., Vincent, P. C., and Clark, S. J. (1999). Concurrent DNA hyper- methylation of multiple genes in acute myeloid leukemia. Cancer Res, 59: 3730-3740.
  • Jackson  P, Millar D, Kingsley  E, Yardley  G, Ow  K, Clark  S, Russell PJ. (2000) Methylation  of a CpG island within the promoter  region  of the KAI1 metastasis suppressor gene  is not  responsible for down-regulation of  KAI1 expression  in invasive cancers or cancer cell lines. Cancer Lett; 157(2):169-76.
  • Millar DS, Paul CL, Molloy PL, Clark SJ. (2000). A distinct sequence (ATAAA)n separates methylated and unmethylated domains at the 5′-end of the GSTP1 CpG island. J Biol Chem; 275(32):24893-9.
  • Paul Jackson, Douglas Millar, Elizabeth Kingsley, Gina Yardley, Kim Ow, Susan Clark, Pamela J Russell (2000).  Methylation of a CpG island within the KAI1 promoter region is not responsible for reduced KAI1 mRNA expression in invasive bladder cancer cell lines.  Cancer Letters 157: 169-176.
  • Melki, J. R., Vincent, P. C., Brown, R. D., and Clark, S. J. (2000). Hypermethylation of E-cadherin in leukemia. Blood, 95: 3208-3213.
  • Welch J, Millar D, Goldman A, Heenan P, Stark M, Eldon M, Clark S, Martin NG, Hayward NK (2001). Lack of genetic and epigenetic changes in CDKN2A in melanocytic nevi. J Invest Dermatol. Aug;117(2):383-4.
  • Chetcuti A, Margan SH, Russell P, Mann S, Millar DS, Clark SJ, Rogers J, Handelsman DJ, Dong Q (2001). Loss of annexin II heavy and light chains in prostate cancer and its precursors. Cancer Res. Sep 1;61(17):6331-4.
  • Schmitt JF, Millar DS, Pedersen JS, Clark SL, Venter DJ, Frydenberg M, Molloy PL, Risbridger GP (2002). Hypermethylation of the inhibin alpha-subunit gene in prostate carcinoma. Mol Endocrinol. Feb;16(2):213-20.
  • Warnecke P, Stirzaker C, Song J, Grunau C, Melki JR, Clark SJ, (2002). Identification and resolution of artifacts in bisulfite sequencing, Methods, 27:101-107.
  • Song, J. Z., Stirzaker, C., Harrison, J., Melki, J. R., and Clark, S. J. (2002). Hypermethylation trigger of the glutathione-S-transferase gene (GSTP1) in prostate cancer cells. Oncogene, 21: 1048-1061.
  • Millar DS, Warnecke PM, Melki JR, Clark SJ. (2002) Methylation sequencing from limiting DNA: embryonic, fixed, and microdissected cells. Methods. Jun;27(2):108-13.
  • Clark, S. J. and Melki, J.R (2002). DNA methylation and gene silencing in cancer: which is the guilty party? Oncogene, 21: 5380-5387.
  • Melki, J. R. and Clark, S. J. (2002). DNA methylation changes in leukae- mia. Semin Cancer Biol, 12: 347-357.
  • Clark SJ, Millar DS, Molloy P. (2003) Bisulfite methylation analysis of tumor suppressor genes in prostate cancer from fresh and archival tissue samples.  Methods Mol Med.;81:219-40.
  • Noble, J. R., Zhong, Z. H., Neumann, A. A., Melki, J. R., Clark, S. J. and Reddel, R. R. (2004). Alterations in the p16(INK4a) and p53 tumor suppressor genes of hTERT-immortalized human fibroblasts. Oncogene, 23:3116-21.
  • Sidhu, S., Martin, E., Gicquel, C., Melki, J., Clark, S. J., Campbell, P., … & Robinson, B. G. (2005). Mutation and methylation analysis of TP53 in adrenal carcinogenesis. European Journal of Surgical Oncology (EJSO), 31(5), 549-554.
  • Hinshelwood RA, Huschtscha LI, Melki J, Stirzaker C, Abdipranoto A, Vissel B, Ravasi T, Wells CA, Hume DA, Reddel RR, Clark SJ. (2007). Concordant epigenetic silencing of transforming growth factor- beta signaling pathway genes occurs early in breast carcino- genesis. Cancer Res. Dec 15;67(24):11517-27.
  • Dahl JA, Duggal S, Coulston N, Millar D, Melki J, Shahdadfar A, Brinchmann JE, Collas P. (2008). Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum. Int J Dev Biol. 52(8):1033-42.
  • Hinshelwood, R. A., Melki, J. R., Huschtscha, L. I., Paul, C., Song, J. Z., Stirzaker, C., Reddel, R.R. & Clark, S. J. (2009). Aberrant de novo methylation of the p16INK4A CpG island is initiated post gene silencing in association with chromatin remodelling and mimics nucleosome positioning. Human molecular genetics, 18(16), 3098-3109.
  • Millar DS, Christova Y, and Holliger P, (2015). A polymerase engineered for bisulfite sequencing. Nucleic Acids Res. 2015 : gkv798v1-gkv798.